The program can be downloaded for a local installation or can be accessed via a web server, where reads can be uploaded for (2016) Fast and sensitive taxonomic classification for metagenomics with Kaiju. Taxonomic classification, using either protein or nucleotide queries. Kaiju can also be used for querying any custom protein database without Per minute using, for example, only 10 GB RAM with a reference database comprising 4821 complete microbial genomes. The search can process up to millions of reads Memory-efficient implementation of the Burrows-Wheeler transform, whichįinds maximum exact matches (MEMs), optionally allowing mismatches in Searched in the database using a modified backward search on a Reads are translated into amino acid sequences, which are then The NCBI BLAST non-redundant protein database nr, optionally also including fungi and microbial eukaryotes. Kaiju can use either the set of available complete genomes from NCBI RefSeq or the microbial subset of High-throughput sequencing reads from metagenomic whole genome sequencing or metatranscriptomics experiments.Įach sequencing read is assigned to a taxon in the NCBI taxonomy by comparing it to a reference database containing microbial and viral protein sequences.īy using protein-level classification, Kaiju achieves a higher sensitivity compared with methods based on nucleotide comparison. Kaiju is a program for sensitive taxonomic classification of

Updated git for mac 2016